Journal
/
/
Syre Glukos Deprivation och ny syresättning som en<em> In Vitro</em> Ischemi-reperfusion skada modell för att studera blodhjärnbarriären dysfunktion
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction
DOI:

08:56 min

May 07, 2015

, , ,

Chapitres

  • 00:05Titre
  • 01:16Seeding Endothelial Cells (RBMECs)
  • 03:31Oxygen and Glucose Deprivation-Reoxygenation In Vitro Model
  • 04:40Immunofluorescence Localization and f-actin Labeling with Rhodamine Phalloidin
  • 07:29An Oxygen-Glucose Deprivation and Reoxygenation (OGD-R) Experiment
  • 08:22Conclusion

Summary

Traduction automatique

Ischemia-Reperfusion (IR) injury is associated with a high rate of morbidity and mortality. The goal of the in vitro model of oxygen-glucose deprivation and reoxygenation (OGD-R) described here is to assess the effects of ischemia reperfusion injury on a variety of cells, particularly in blood-brain barrier (BBB) endothelial cells.

Vidéos Connexes

Read Article