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Xenopus laevis som en model til at identificere Oversættelse Nedskrivning
Journal JoVE
Biologie
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Journal JoVE Biologie
Xenopus laevis as a Model to Identify Translation Impairment
DOI:

10:24 min

September 27, 2015

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Chapitres

  • 00:05Titre
  • 02:39mRNA Synthesis
  • 03:55Microinjection of Synthesized RNA and Stimulation of Oocyte Maturation
  • 05:15Germinal Vesicle Breakdown and Western Blot Analysis
  • 06:21Polyadenylation Assay
  • 08:08Results: Xenopus Oocyte Translation Analysis on Maturation
  • 09:43Conclusion

Summary

Traduction automatique

Protein synthesis control occurs mainly at the translation initiation step, deficiencies in which are linked to diverse disorders. To better understand their etiology, we described here a protocol using Xenopus laevis oocytes assessing the translation of mos transcript in the presence of a mutant of translation initiation factor eIF4G1.

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