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Testen Bloedcel Populaties van de<em> Drosophila melanogaster</em> Larva
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Assaying Blood Cell Populations of the Drosophila melanogaster Larva
DOI:

11:15 min

November 11, 2015

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Chapitres

  • 00:05Titre
  • 03:31Larvae Collection and Dissection
  • 05:58Quantification
  • 07:30Hemocyte Disturbance Assay
  • 09:03Results: Transient Changes in Circulating and Resident Hemocyte Populations
  • 09:54Conclusion

Summary

Traduction automatique

Drosophila blood cells, or hemocytes, cycle between resident sites and circulation. In the larva, resident (sessile) hemocytes localize to inductive microenvironments, the Hematopoietic Pockets, while circulating hemocytes move freely in the hemolymph. The goal of this protocol is the standardized isolation and quantification of these two, behaviorally distinct but interchanging, hemocyte populations.

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