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Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta
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Journal JoVE Biologie
Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta

Two-photon Imaging of Intracellular Ca2+ Handling and Nitric Oxide Production in Endothelial and Smooth Muscle Cells of an Isolated Rat Aorta

DOI:
10.3791/52734-v

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08:08 min

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June 10, 2015

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, , , ,
1Departments of Physiology,Medical College of Wisconsin, 2Human and Molecular Genetics Center,Medical College of Wisconsin, 3Cardiovascular Center,Medical College of Wisconsin, 4Blood Research Institute of Wisconsin

Chapitres

  • 00:05Titre
  • 02:20Preparation of Rat Aorta, Dye Loading, and Incubation
  • 03:26Laser Scanning Two Photon Microscopy Protocol
  • 04:39Image Processing and Calculations
  • 05:55Results: Intracellular Calcium in the Rat Aorta
  • 07:08Conclusion

Summary

Traduction automatique

Traduction automatique

Vascular cell functiondepends on activity of intracellular messengers. Described here is an ex vivo two photon imaging method that allows the measurement of intracellular calcium and nitric oxide levels in response to physiological and pharmacological stimuli in individual endothelial and smooth muscle cells of an isolated aorta.

Tags

Keywords: Two-photon ImagingIntracellular Ca2+Nitric OxideEndothelial CellsSmooth Muscle CellsIsolated Rat AortaCalcium HandlingNO ProductionVascular Function

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