Journal
/
Bio-ingénierie
/
Dyrkning Mus hjerteklapper i Miniature Tissue Culture System
Journal JoVE
Bio-ingénierie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Bio-ingénierie
Culturing Mouse Cardiac Valves in the Miniature Tissue Culture System

Dyrkning Mus hjerteklapper i Miniature Tissue Culture System

DOI:
10.3791/52750-v

•

08:47 min

•

October 19, 2015

•

, , , ,
1Department of Molecular Cell Biology,Leiden University Medical Center, 2Department of Engineering Technology,New Jersey Institute of Technology, 3Department of Urology,Leiden University Medical Center, 4Cardiovascular Research Institute, Department of Cell Biology and Molecular Medicine,Rutgers New Jersey Medical School

Chapitres

  • 00:05Titre
  • 01:05Miniature Tissue Culture System (MTCS) Assembly
  • 02:13Mouse Heart Isolation
  • 04:01Mouse Heart Cannulation in the Perfusion Chamber
  • 05:21Mitral and Aortic Valve Ligation and Culture
  • 07:16Results: Histological Analysis of Cultured Valves from 2 Month Old Mice
  • 08:19Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we present an ex vivo flow model in which murine cardiac valves can be cultured allowing the study of the biology of the valve.

Tags

Keywords: Mouse Cardiac ValvesHeart Valve DiseaseValvular Biology3D OrganizationValvular Endothelial CellsValvular Interstitial CellsEx Vivo Flow ModelMitral ValveAortic ValveValvular CellsSignaling Pathways

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/52750?article_type=v

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System
check_url/fr/52750?article_type=v

Encapsulation of Cardiomyocytes in a Fibrin Hydrogel for Cardiac Tissue Engineering
check_url/fr/52750?article_type=v

Kapillarkraft Litografi for Cardiac Tissue Engineering
check_url/fr/52750?article_type=v

Anatomiske rekonstruktioner af Human Cardiac Venøs System ved hjælp Kontrast-computertomografi af Perfusion-faste Prøver
check_url/fr/52750?article_type=v

Protokol for Relativ Hydrodynamic Vurdering af Tri-brochure Polymer Valves
check_url/fr/52750?article_type=v

Tissue Engineering af et menneske 3D<em> In vitro</em> Tumor Test System
check_url/fr/52750?article_type=v

Generation af en tre-dimensionel fuld tykkelse Skin Equivalent og Automated Anskydning
check_url/fr/52750?article_type=v

en<em> In vitro</em> Orgel Kultur Model af muse diskusprolaps
check_url/fr/52750?article_type=v

Dyrkning pattedyrsceller i tre-dimensionelle peptid stilladser
check_url/fr/52750?article_type=v

En netto skimmel-baseret metode til stillads-gratis tre-dimensionelle hjerte væv skabelse

Read Article