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Scalable Platte mit 96 Vertiefungen Basierend iPSC Kultur und Produktion Mit einem Robotic Flüssigkeitshandhabungssystem
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Chapitres
- 00:05Titre
- 01:54Feeding 96-well Plate Stem Cell Colonies
- 03:26Passaging 96-well Plate Stem Cell Colonies
- 06:03Results: Representative Plate Based Robotic Stem Cell Culture
- 07:36Conclusion
The protocols described allow laboratories to perform scalable, adherent stem cell culture in high throughput with minimal labor, experience and equipment investment cost using a programmable liquid handling robot and 96-well plates. iPSCs passaged more than 20 times on this system maintained pluripotency, normal karyotypes and differentiated into cardiomyocytes.
