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Recherche en cancérologie
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Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors
Journal JoVE
Recherche en cancérologie
This content is Free Access.
Journal JoVE Recherche en cancérologie
Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

DOI:
10.3791/52758-v

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11:15 min

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September 20, 2016

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, , , , , , , , , , , ,
1Department of Pathology and Laboratory Medicine,Perelman School of Medicine, University of Pennsylvania, 2Division of Hematology/Oncology, Department of Medicine,Perelman School of Medicine, University of Pennsylvania, 3Abramson Cancer Center

Chapitres

  • 00:05Titre
  • 01:19Genomic DNA Quality Control and Amplicon Library Preparation
  • 03:23Indexing and PCR Amplification
  • 04:37PCR Purification and Bead-based Normalization
  • 06:33Sequencing and Data Analysis
  • 07:19Results: Mutations Detected in Solid and Liquid Tumors Using Next Generation Sequencing
  • 09:06Conclusion

Summary

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Traduction automatique

This manuscript describes clinical protocols for two next-generation sequencing panels. One panel interrogates hematologic malignancies while the other panel targets genes commonly mutated in solid tumors. Molecular classification of driver mutations in human malignancies offers valuable prognostic and predictive information.

Tags

Next Generation SequencingTargeted Amplicon AssayActionable MutationsSolid TumorsLiquid TumorsTherapeutic UseDiagnosticPrognosticResponse To Targeted TherapyGene MutationsMedical OncologyGenomic DNAAmplicon LibraryOligo HybridizationFilter Plate UnitExtension Ligation

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