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Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
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Journal JoVE Biologie
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

DOI:
10.3791/52824-v

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07:48 min

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July 03, 2015

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,
1Department of Anesthesiology and Perioperative Care,University of California Irvine, 2Department of Biomedical and Molecular Sciences,Queen’s University, 3Department of Pharmacology,University of California Irvine

Chapitres

  • 00:05Titre
  • 01:25Drug Treatment of Cultured Cells and Immunocytochemistry
  • 03:26Microscope Settings and Imaging
  • 04:57Colocalization Analysis and Data Normalization
  • 06:17Results: Representative Photomicrographs of Receptor and Compartment-marker Labeling with False-color Colocalization Maps
  • 07:18Conclusion

Summary

Traduction automatique

Traduction automatique

Receptor trafficking modulates signaling and cell responsiveness to ligands and is, itself, responsive to cell conditions, including ligand-induced signaling. Here, we describe a powerful and flexible technique for quantitatively assessing drug-induced receptor trafficking using immunolabeling and colocalizational analysis.

Tags

Receptor TraffickingReceptor InternalizationColocalization AnalysisImmunolabelingConfocal MicroscopyDrug-induced ChangesCell SignalingIntracellular TraffickingQuantitative Analysis

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