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Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury
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Journal JoVE Médecine
Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury

Triggering Reactive Gliosis In Vivo by a Forebrain Stab Injury

DOI:
10.3791/52825-v

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07:46 min

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June 29, 2015

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1Department of Biology,Drexel University

Chapitres

  • 00:05Titre
  • 01:21Preparing Surgical Area
  • 02:06Surgical Procedure
  • 06:12Results: Immunohistology of the Lesion After Forebrain Stab Injury
  • 07:21Conclusion

Summary

Traduction automatique

Traduction automatique

This article describes a detailed protocol to produce a forebrain stab injury in adult mice. The stab injury induces severe reactive gliosis and glial scar formation which can be subsequently examined by standard immunohistochemistry methods.

Tags

Keywords: Reactive GliosisAstrocytesCNS InjuryForebrain Stab InjuryNeuroinflammationBlood-brain BarrierNeural Repair

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