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Ein Aquatic Microbial Metaproteomik Workflow: Von der Zelle zum tryptischen Peptide Geeignet für Tandem-Massenspektrometrie-basierte Analyse
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Journal JoVE Environnement
An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis
DOI:

08:09 min

September 15, 2015

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Chapitres

  • 00:05Titre
  • 01:06Perform Cell Lysis in Catridge Filter Unit with Cells Preserved in RNA Stabilization Solution
  • 04:07Protein Precipitation and Peptide Extraction
  • 05:25DNA Precipitation
  • 06:49Results: Taxonomic and Functional Analysis of Arctic Ocean Microbial Communities
  • 07:50Conclusion

Summary

Traduction automatique

This protocol is for the extraction and concentration of protein and DNA from microbial biomass collected from seawater, followed by the generation of tryptic peptides suitable for tandem mass spectrometry-based proteomic analysis.

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