Journal
/
Immunologie et infection
/
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA

DOI:
10.3791/52836-v

•

11:15 min

•

May 21, 2015

•

, , ,
1Department of Oral Microbiology and Immunology,School of Dentistry and Dental Research Institute, Seoul National University

Chapitres

  • 00:05Titre
  • 01:35Making the Probe
  • 04:02In Situ Hybridization
  • 06:53Applying an Antibody and Counter Stain
  • 08:56Results: Comparing Eubacterial and P. gingivalis-specific Probes and Using the Eubacterial Probe for General Bacterial Detection Purposes
  • 10:32Conclusion

Summary

Traduction automatique

Traduction automatique

Here a method to localize bacteria within paraffin-embedded tissues using DIG-labeled 16S rRNA-targeting DNA probes has been described. This protocol can be applied to study the role of bacteria in various diseases such as periodontitis, cancers, and inflammatory immune diseases.

Tags

Keywords: In Situ DetectionBacteriaDigoxin-labeled DNA Probe16S RRNAPeriodontitisGingival BiopsiesElectron MicroscopyImmunohistochemistryImmunofluorescenceFluorescent In Situ Hybridization (FISH)Species-specificUniversalBacterial FormsEpitheliaInflammatory InfiltratesBlood VesselsPeriodontitisCancerInflammatory Immune Diseases

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/52836?article_type=v

Combination of Adhesive-tape-based Sampling and Fluorescence in situ Hybridization for Rapid Detection of Salmonella on Fresh Produce
check_url/fr/52836?article_type=v

ampliPHOX Colorimetric Detection on a DNA Microarray for Influenza
check_url/fr/52836?article_type=v

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres
check_url/fr/52836?article_type=v

Detection of Bacteria Using Fluorogenic DNAzymes
check_url/fr/52836?article_type=v

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes
check_url/fr/52836?article_type=v

Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues
check_url/fr/52836?article_type=v

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry
check_url/fr/52836?article_type=v

In Situ Detection of Autoreactive CD4 T Cells in Brain and Heart Using Major Histocompatibility Complex Class II Dextramers
check_url/fr/52836?article_type=v

A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
check_url/fr/52836?article_type=v

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS

Read Article