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Iniezioni intramuscolari Lungo le piastre Motor finale: un approccio mini-invasivo di navetta Tracers Direttamente nella Motor Neurons
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Intramuscular Injections Along the Motor End Plates: A Minimally Invasive Approach to Shuttle Tracers Directly into Motor Neurons
DOI:

10:57 min

July 13, 2015

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Chapitres

  • 00:05Titre
  • 01:58Acetylcholinesterase Histochemical Staining
  • 02:49Intramuscular Injections at the Motor End Plates
  • 04:49Cervical Spinal Cord Dissection and Histology Preparation
  • 07:35Lumbar Spinal Cord Dissection and Histology Preparation
  • 08:49Results: Representative Motor End Plate Staining and Analysis
  • 10:16Conclusion

Summary

Traduction automatique

The efficacy of intramuscular uptake and retrograde transport of molecules to corresponding motor neurons depends on the location of the injection sites with respect to the motor end plates (MEPs). Here, we describe how to locate MEPs on skeletal muscles to optimise retrograde transport of tracers into motor neurons.

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