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Isolierung von Leukozyten aus dem Mausgeweben an der Maternal-Fetal-Schnittstelle
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1Department of Obstetrics & Gynecology,Wayne State University School of Medicine, 2School of Paediatrics and Reproductive Health, Research Centre for Reproductive Health, the Robinson Research Institute,The University of Adelaide, 3Department of Immunology & Microbiology,Wayne State University School of Medicine, 4Perinatology Research Branch,NICHD/NIH/DHHS
Chapitres
- 00:05Titre
- 01:23Tissue Collection
- 04:00Uterine, Decidual, and Placental Tissue Dissociation
- 05:58Results: Leukocyte Populations in the Maternal-fetal Interface
- 07:01Conclusion
Described herein is a protocol to isolate and analyze the infiltrating leukocytes of tissues at the maternal-fetal interface (uterus, decidua, and placenta) of mice. This protocol maintains the integrity of most cell surface markers and yields enough viable cells for downstream applications including flow cytometry analysis.
