Реализация<em> In Vitro</em> Лекарственной устойчивости Анализы: Максимизация потенциала для раскрытия клинически значимых механизмов сопротивления

Drug resistance to targeted therapeutics is widespread and the need to identify mechanisms of resistance--prior to or following clinical onset--is critical for guiding alternative clinical management strategies. Here, we present a protocol to couple derivation of drug-resistant lines in vitro with sequencing to expedite discovery of these mechanisms.