/
/
Immunohistochemical Visualization of Hippocampal Neuron Activity After Spatial Learning in a Mouse Model of Neurodevelopmental Disorders
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:30Preparation of Brain Sections
- 02:16pERK Immunohistochemistry
- 05:22Bright-field Imaging of Neuron Activation
- 05:43Results: pERK is a Reliable Molecular Readout of Learning Dependent Neuronal Activation
- 06:45Conclusion
We describe an immunohistochemistry protocol to study the profile of hippocampal neuron activation following exposure to a spatial learning task in a mouse model characterized by cognitive deficits of neurodevelopmental origin. This protocol can be applied to both genetic or pharmacological mouse models characterized by cognitive deficits.
