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प्लानर ढाल प्रसार प्रणाली एक 3 डी कोलेजन मैट्रिक्स में कीमोटैक्सिस जांच
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix
DOI:

09:26 min

June 12, 2015

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Chapitres

  • 00:05Titre
  • 01:17Hydrophillic Glass Coverslips Preparation
  • 03:00Hydrophobic Glass Coverslips Preparation
  • 03:493D Silicone Mold Assembly
  • 05:43Creation of Collagen Mixture and 3D Matrix
  • 07:53Results: Chemoattractants Used in Planar Gradient Diffusion System Directs Cell Movement through Collagen Matrix
  • 09:03Conclusion

Summary

Traduction automatique

Cell migration is an important part of human development and life. In order to understand the mechanisms that can alter cell migration, we present a planar gradient diffusion system to investigate chemotaxis in a 3D collagen matrix, which allows one to overcome modern diffusion chamber limitations of existing assays.

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