Waiting
Traitement de la connexion…

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Production of Nurr-1 Specific Polyclonal…
JoVE Journal
Biology

Un abonnement à JoVE est nécessaire pour afficher ce contenu. Connectez-vous ou commencez votre essai gratuit.

JoVE Journal Biology
Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77
 

Production of Nurr-1 Specific Polyclonal Antibodies Free of Cross-reactivity Against Its Close Homologs, Nor1 and Nur77

Article DOI: 10.3791/52963-v • 12:30 min • August 17th, 2015
August 17th, 2015 •
1, 1,2, 1, 1, 1, 1
1Molecular Neurobiology Laboratory, Psychiatry, McLean Hospital, 2Department of Biochemistry, College of Medicine, Konyang University

Chapitres

résumé
Automatic Translation

Here, we selectively target antibodies against a specific member of a highly conserved family of proteins by immunizing animals with their most divergent regions followed by removing cross reactive antibodies by pre-adsorption.

Tags

Nurr-1 Polyclonal Antibodies Cross-reactivity Nor1 Nur77 Nuclear Receptor Subfamily 4 DNA Binding Domain Ligand-binding Domain Dopaminergic System Dopamine Dysfunctions Parkinson's Disease Schizophrenia Manic Depression Hippocampus Learning And Memory Nerve Growth Factor IB Neuron-derived Orphan Receptor 1
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter