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Instillation and Fixation Methods Useful in Mouse Lung Cancer Research
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Journal JoVE Médecine
Instillation and Fixation Methods Useful in Mouse Lung Cancer Research

Instillation and Fixation Methods Useful in Mouse Lung Cancer Research

DOI:
10.3791/52964-v

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06:57 min

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August 31, 2015

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, ,
1Bloomberg School of Public Health, Environmental Health Sciences,Johns Hopkins University, 2Department of Medicine, Pulmonary Diseases and Critical Care Medicine,University of North Carolina School of Medicine

Chapitres

  • 00:05Titre
  • 01:51Lung Instillation
  • 03:34Lung Fixation
  • 04:55Measurement of the Fixed Lung Volume
  • 06:01Results: Instillation and Fixed Lung Volume Measurement
  • 06:21Conclusion

Summary

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Traduction automatique

The goal of this paper is to describe simple methods that will greatly aid in the setup and analysis of mouse lungs with lung cancer or other pathologies. We present 3 protocols to simply and reliably carry out lung instillations, fixation, and lung volume measurements.

Tags

Mouse Lung Cancer ResearchTracheal InstillationLung FixationLung Volume MeasurementHistologic AnalysisStereological QuantificationLung StructureLung Tumor

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