/
/
Mikrofluidischen Genipin Abscheidetechnik für Extended Kultur von mikrostrukturierten Vascular Muscular Thin Films
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 02:11Poly(N-iso-propylacrylamide) Strip Coating of Coverslips
- 03:38Microfluidic Patterning for Engineering Tissues
- 04:57Microfluidic Device Preparation and Deposition of Genipin and Fibronectin
- 07:14Smooth Muscle Cell Seeding and vMTF Contractility Experiment
- 09:15Results: Retention of Native Tissue Structure and Function in Vascular Muscular Thin Films
- 11:06Conclusion
We present a method for microfluidic deposition of patterned genipin and fibronectin on PDMS substrates, allowing extended viability of vascular smooth muscle cell-dense tissues. This tissue fabrication method is combined with previous vascular muscular thin film technology to measure vascular contractility over disease-relevant time courses.
