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利用酶生物传感器的量化内源性ATP或H<sub> 2</sub> 0<sub> 2</sub>在肾脏

Journal JoVE
Biologie

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Journal JoVE Biologie

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H₂O₂ in the Kidney

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利用酶生物传感器的量化内源性ATP或H<sub> 2</sub> 0<sub> 2</sub>在肾脏

DOI:

10.3791/53059-v

•

10:00 min

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October 12, 2015

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Oleg Palygin, Vladislav Levchenko, Louise C. Evans, Gregory Blass, Allen W. Cowley Jr., Alexander Staruschenko

¹Department of Physiology,Medical College of Wisconsin

Chapitres

00:05Titre
02:03Sensor Calibration
04:38Animal Surgeries
06:09Data Acquisition Ex Vivo
07:23Results: Microelectrode Biosensor Detection of Analytes in Whole Kidneys
08:57Conclusion

Summary

Traduction automatique

Enzymatic microelectrode biosensors enable real-time measurements of extracellular cell signaling in biologically-relevant concentrations. The following protocols extend the applications of biosensors to the ex vivo and in vivo detection of ATP and H₂O₂ in the kidney.

Tags

Enzymatic Biosensors ATP H2O2 Kidney Real-time Detection Amperometry Glycerol Kinase Glycerol-3-phosphate Oxidase Platinum/iridium Electrode Gold Electrode Catalase Apyrase Calibration Ex Vivo In Vivo

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