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Biologie du développement
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Method of Studying Palatal Fusion using Static Organ Culture
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Biologie du développement
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Journal JoVE Biologie du développement
Method of Studying Palatal Fusion using Static Organ Culture

Method of Studying Palatal Fusion using Static Organ Culture

DOI:
10.3791/53063-v

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04:58 min

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September 19, 2015

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, ,
1Department of Biomedical Sciences and Center for Craniofacial Research and Diagnosis,Texas A&M University Baylor College of Dentistry

Chapitres

  • 00:05Titre
  • 01:09Mouse Embryo Collection
  • 02:03Palate Dissection and Culture
  • 03:38Results: The Effects of Nicotine Treatment on Murine Palatal Fusion
  • 04:30Conclusion

Summary

Traduction automatique

Traduction automatique

Studies of palate development are motivated by the incidence of cleft palate, a birth defect that imposes a tremendous health burden and can leave lasting disfigurement. We demonstrate here a technique to culture palatal shelves that can be used to study different signaling pathways involved in palatal development and fusion.

Tags

Keywords: Palatal FusionStatic Organ CultureCleft Lip And PalateMesenchymal ShelvesMidline Epithelial SeamEpithelial To Mesenchymal TransitionApoptosisPalatal ConfluenceIn Vitro ProtocolExogenous Chemical AgentsRegulatory FactorsGrowth FactorsProteins

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