Journal
/
/
En organotypiske høy gjennomstrømning System for Karakterisering av Drug Sensitivity of Primary myelomatose Cells
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells
DOI:

09:41 min

July 15, 2015

, , , ,

Chapitres

  • 00:05Titre
  • 01:37Plate Seeding with a Manual Multiple-channel Pipetter
  • 02:33Plate Seeding with a Robotic Pipetter
  • 05:23Plate Drugging Using a Robotic Pipettor and Seeded Cell Imaging
  • 07:36Results: Representative Robotic Seeding and Drug Treatment of MM Cells
  • 09:01Conclusion

Summary

Traduction automatique

We describe a high-throughput drug sensitivity assay for primary multiple myeloma cells. It consists of a reconstruction of the bone marrow microenvironment (including extracellular matrix and stroma) in multi-well plates, and a non-invasive method for longitudinal quantification of cell viability.

Vidéos Connexes

Read Article