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Electrophysiology on Isolated Brainstem-spinal Cord Preparations from Newborn Rodents Allows Neural Respiratory Network Output Recording
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Journal JoVE Neurosciences
Electrophysiology on Isolated Brainstem-spinal Cord Preparations from Newborn Rodents Allows Neural Respiratory Network Output Recording

Electrophysiology on Isolated Brainstem-spinal Cord Preparations from Newborn Rodents Allows Neural Respiratory Network Output Recording

DOI:
10.3791/53071-v

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05:28 min

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November 19, 2015

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,
1Department of Pediatrics,Laval University

Chapitres

  • 00:05Titre
  • 01:11Dissection
  • 02:56Recording
  • 04:26Results: Examples of Recordings Obtained from Brainstem-spinal Cord Preparations
  • 04:59Conclusion

Summary

Traduction automatique

Traduction automatique

The central respiratory drive is located in the brainstem. Spontaneous respiratory motor output from an isolated brainstem-spinal cord is recorded by placing an electrode on the fourth ventral root. This experimental approach is valuable for pharmacological investigations or the assessment of respiratory challenges and genetic manipulations on rhythmic motor behavior.

Tags

ElectrophysiologyBrainstem-spinal Cord PreparationNewborn RodentsNeural Respiratory NetworkRespiratory DriveBrainstemVentral Root RecordingInspiratory SignalPhrenic NerveBurst Analysis

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