/
/
طبقة تلو طبقة الكولاجين ترسب في أجهزة ميكروفلويديك لMicrotissue لتحقيق الاستقرار
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:39Collagen Methylation
- 03:52Collagen Succinylation
- 06:17Layer-by-layer Collagen Deposition
- 07:56Results: Layer-by-layer Collagen Deposition Stabilizes Hepatocytes in Microfluidic Devices
- 09:22Conclusion
The creation of functional microtissues within microfluidic devices requires the stabilization of cell phenotypes by adapting traditional cell culture techniques to the limited spatial dimensions in microdevices. Modification of collagen allows the layer-by-layer deposition of ultrathin collagen assemblies that can stabilize primary cells, such as hepatocytes, as microfluidic tissue models.
