Journal
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Microenvironments-شطيرة مثل لتسخير خلية / المواد التفاعلات
Journal JoVE
Bio-ingénierie
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Journal JoVE Bio-ingénierie
Sandwich-like Microenvironments to Harness Cell/Material Interactions
DOI:

06:50 min

August 04, 2015

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Chapitres

  • 00:05Titre
  • 01:27Production of Dorsal Substrates
  • 02:52Sandwich Culture
  • 03:55Wound Healing Assay
  • 04:55Results: Sandwich-like Culture Strongly Influences Cell Morphology, Cell Differentiation and ECM Reorganization
  • 06:18Conclusion

Summary

Traduction automatique

The following protocol describes the procedure to assemble sandwich-like cultures to be used as an intermediate stage between bi-dimensional (2D) and three-dimensional (3D) cellular environments. The engineered systems can have applications in microscopy, biomechanics, biochemistry and cell biology assays.

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