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Et hormon-responsive 3D Culture Model of Human melkekjertlene Epitel
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Journal JoVE Bio-ingénierie
A Hormone-responsive 3D Culture Model of the Human Mammary Gland Epithelium

Et hormon-responsive 3D Culture Model of Human melkekjertlene Epitel

DOI:
10.3791/53098-v

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08:24 min

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February 07, 2016

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, , ,
1Department of Integrative Physiology and Pathobiology,Tufts University School of Medicine

Chapitres

  • 00:05Titre
  • 01:04T47D Cell 3D Culture
  • 03:02Whole Mount Gel Processing
  • 04:24Gel Processing for Histological Analysis
  • 05:46Cell Extraction and Quantification
  • 06:39Results: Representative 3D Cell Cultures
  • 07:48Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a 3D culture model of the human breast epithelium that is suitable to study hormone action.

Tags

Keywords: 3D Culture ModelHuman Mammary Gland EpitheliumHormone-responsiveMammogenic HormonesMammary Epithelial MorphogenesisEstrogenProgesteroneProlactinBreast Cancer TherapyT47D CellsCollagen GelCdFBS Medium3D Culture Processing

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