Journal
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Fare Kolon Mukozanın Mikrodisseksiyon için Cryosectioning Yöntemi
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Cryosectioning Method for Microdissection of Murine Colonic Mucosa
DOI:

06:16 min

July 12, 2015

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Chapitres

  • 00:05Titre
  • 01:07Preparation for Cryosectioning
  • 02:04Dissection of Distal Colonic Tissue
  • 02:51Mounting and Cryosectioning the Tissue
  • 04:15Results: Morphology of Sectioned Tissue
  • 05:55Conclusion

Summary

Traduction automatique

Here we describe a simple method for the isolation of spatially distinct murine colonic epithelial surface cells from the underlying crypt-base cells.

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