Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 00:53Preparing Tubule Transfer Rods
- 01:33Preparing the Ion-Specific Electrodes
- 03:38Preparing the Reference Electrodes and Calibrating the Electrodes
- 07:16Tubule Dissection
- 09:41Making Measurements
- 11:06Results: Measurement K+ and Na+ Ion Fluxes to Investigate the Role of the Sodium-potassium-2-chloride Cotransporter (NKCC)
- 12:07Conclusion
This protocol describes the use of the Ramsay assay to measure fluid secretion rates from isolated Malpighian (renal) tubules from Drosophila melanogaster. In addition, the use of ion-specific electrodes to measure sodium and potassium concentrations in the secreted fluid, allowing calculation of transepithelial ion flux, is described.
