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Anvendelse af Ramsay-analysen til at måle Fluid sekretion og Ion Flux priser i<em> Drosophila melanogaster</em> Malpighian tubulus
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Journal JoVE Biologie
Use of the Ramsay Assay to Measure Fluid Secretion and Ion Flux Rates in the Drosophila melanogaster Malpighian Tubule

Anvendelse af Ramsay-analysen til at måle Fluid sekretion og Ion Flux priser i<em> Drosophila melanogaster</em> Malpighian tubulus

DOI:
10.3791/53144-v

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13:30 min

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November 25, 2015

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,
1Department of Internal Medicine,University of Texas Southwestern Medical Center

Chapitres

  • 00:05Titre
  • 00:53Preparing Tubule Transfer Rods
  • 01:33Preparing the Ion-Specific Electrodes
  • 03:38Preparing the Reference Electrodes and Calibrating the Electrodes
  • 07:16Tubule Dissection
  • 09:41Making Measurements
  • 11:06Results: Measurement K+ and Na+ Ion Fluxes to Investigate the Role of the Sodium-potassium-2-chloride Cotransporter (NKCC)
  • 12:07Conclusion

Summary

Traduction automatique

Traduction automatique

Denne protokol beskriver anvendelsen af Ramsay assay til måling af væske sekretionshastigheder fra isolerede Malpighian (renal) tubuli fra Drosophila melanogaster. Desuden anvendelsen af ​​ionspecifikke elektroder måler natrium og kalium koncentrationer i den udskilte væske, tillader beregning af transepitel ion flux, er beskrevet.

Tags

Ramsay AssayFluid SecretionIon FluxDrosophila MelanogasterMalpighian TubuleEpithelial Ion TransportTransepithelial FluidIon-specific ElectrodesSodiumPotassiumGénétiqueMolecular MechanismsHomeostasis

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