/
/
Preparation of Mitochondrial Enriched Fractions for Metabolic Analysis in Drosophila
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:12Fly Food and Media Preparation
- 02:08Drosophila Strain Rearing
- 04:18Isolation of Mitochondrial Fractions
- 06:13Results: Minimal Contamination of Cytosolic Proteins
- 07:51Conclusion
Mitochondria play central roles in the regulation of metabolism and homeostasis. Subtle changes in mitochondrial metabolism that affect organismal physiology could be difficult to detect in whole organism metabolomics studies. Here we describe an isolation method that enhances the detection of subtle metabolic shifts in Drosophila melanogaster.
