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Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)
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Journal JoVE Bio-ingénierie
Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

Photoactivated Localization Microscopy with Bimolecular Fluorescence Complementation (BiFC-PALM)

DOI:
10.3791/53154-v

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12:42 min

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December 22, 2015

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, , ,
1Department of Biomedical Engineering,Oregon Health and Science University, 2Knight Cancer Institute,Oregon Health and Science University, 3OHSU Center for Spatial Systems Biomedicine,Oregon Health and Science University

Chapitres

  • 00:05Titre
  • 00:40Imaging Fixed Cells
  • 03:57Image Processing
  • 07:46Track Single Molecules in Live Cells
  • 08:55Results: Visualization of Individual Ras-Raf Interactions Using BiFC-PALM
  • 11:02Conclusion

Summary

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Traduction automatique

Protein-protein interactions are visualized in cells with nanometer spatial resolution by combining bimolecular fluorescence complementation (BiFC) with photoactivated localization microscopy (PALM). Described here is the use of BiFC-PALM for imaging Ras-Raf interactions in U2OS cells for visualizing the nanoscale clustering and diffusion of individual Ras-Raf complexes.

Tags

Protein-protein InteractionsBiFC-PALMSuperresolution MicroscopyPhotoactivated Localization MicroscopyBimolecular Fluorescence ComplementationSingle Molecule ImagingRas-Raf Interaction

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