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Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT
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Journal JoVE Bio-ingénierie
Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

Delivery of Proteins, Peptides or Cell-impermeable Small Molecules into Live Cells by Incubation with the Endosomolytic Reagent dfTAT

DOI:
10.3791/53175-v

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10:30 min

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September 02, 2015

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, ,
1Biochemistry and Biophysics,Texas A&M University

Chapitres

  • 00:05Titre
  • 01:53SPPS: CK(TMR)TATG(fTAT) Synthesis
  • 05:58Oxidation Reaction: dfTAT Generation & Measuring dfTAT Concentration
  • 07:40Cellular Delivery Experiments
  • 08:41Results: dfTAT has a High Endosomolytic Activity and can be used for the Delivery of Macromolecules Inside Live Cells
  • 10:03Conclusion

Summary

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Traduction automatique

We describe how to deliver proteins and cell-impermeable small molecules into cultured mammalian cells by a simple co-incubation protocol with a reagent that causes endocytic organelles to become leaky.

Tags

Protein DeliveryPeptide DeliveryCell-impermeable Small Molecule DeliveryEndosomolytic ReagentDfTATCell-penetrating PeptideCytosolic DeliveryMacromolecular DeliveryCell-based AssaysEx Vivo Cell Manipulation

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