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La identificación de patógenos bacterianos raras por 16S rRNA gen secuenciación y MALDI-TOF MS
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Chapitres
- 00:05Titre
- 01:12Preparation of Bacteria for MALDI-TOF MS Analysis
- 02:05Performing the MALDI-TOF MS Analysis
- 04:18Results: MALDI-TOF Spectra and Assigned Scores of Rare Pathogens
- 05:03Conclusion
Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) and molecular techniques (16S rRNA gene sequencing) permit the identification of rare bacterial pathogens in routine diagnostics. The goal of this protocol lies in the combination of both techniques which leads to more accurate and reliable data.
