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Analisi delle larve Zebrafish muscolo scheletrico Integrità con Blu di Evans Dye
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
DOI:

07:34 min

November 30, 2015

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Chapitres

  • 00:05Titre
  • 01:14Preparation of Agar Injection Plates and Evans Blue Dye Injection Mix
  • 02:42Evans Blue Dye Injection Preparation
  • 03:54Pericardial Injection and Uptake of Evans Blue Dye in Zebrafish Larvae
  • 05:29Results: Skeletal Muscle Analysis in Zebrafish Larvae Using Evans Blue Dye
  • 06:14Conclusion

Summary

Traduction automatique

In this study, we describe a straightforward method to perform Evans Blue Dye (EBD) analysis on zebrafish larvae. This technique is a powerful tool for the characterization of skeletal muscle integrity and delineation of zebrafish models of muscular dystrophy, and is a valuable method for the development of novel therapeutics.

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