Endoplasmic reticulum calcium homeostasis is disrupted in diverse pathologies. A secreted ER calcium monitoring protein (SERCaMP) reporter can be used to detect disruptions in the ER calcium store. This protocol describes the use of a Gaussia luciferase SERCaMP to examine ER calcium homeostasis in vitro and in vivo.

Monitoring Endoplasmic Reticulum Calcium Homeostasis Using a Gaussia Luciferase SERCaMP

•

•

•

Chapitres

Summary

Traduction automatique

Tags

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Studying Membrane Biogenesis with a Luciferase-Based Reporter Gene Assay

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Visualization of Endoplasmic Reticulum Localized mRNAs in Mammalian Cells

High-throughput Functional Screening using a Homemade Dual-glow Luciferase Assay

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Transfecting RAW264.7 Cells with a Luciferase Reporter Gene

Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

Read Article