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Caspase-3活性在鼠杏仁核测量用荧光分析法心肌梗死后
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Caspase-3 Activity in the Rat Amygdala Measured by Spectrofluorometry After Myocardial Infarction
DOI:

08:41 min

January 12, 2016

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Chapitres

  • 00:05Titre
  • 00:52Myocardial Infarction Surgery
  • 03:36Isolation of the Amygdala
  • 05:23Caspase-3 Activity Assay
  • 07:39Results: Caspase-3 Activity in the Amygdala of Rats After Myocardial Infarction
  • 08:08Conclusion

Summary

Traduction automatique

Myocardial infarction (MI) is not only followed by impaired cardiac function but also by apoptosis in the amygdala, a brain region involved in the behavioral consequences of MI. This protocol describes how to induce MI, collect amygdala tissue and measure caspase-3 activity, a marker of apoptosis, therein.

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