Journal
/
/
Yüksek verim Mikroplate Solunum Ölçümler Fare İskelet Kası Minimal Miktarları gelen kondriyayı İzole kullanma
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Using Isolated Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High throughput Microplate Respiratory Measurements
DOI:

10:39 min

October 30, 2015

, , , , ,

Chapitres

  • 00:05Titre
  • 01:30Setup
  • 02:53Running the Assay
  • 07:45Results: Microplate Based Assay Measures Oxygen Consumption in Skeletal Muscle Mitochodria
  • 10:05Conclusion

Summary

Traduction automatique

The methods presented provide step-by-step instructions for the performance of a collection of microplate based respirometric assays using isolated mitochondria from minimal quantities of mouse skeletal muscle. These assays are able to measure mechanistic changes/adaptations in mitochondrial oxygen consumption in a commonly used animal model.

Vidéos Connexes

Read Article