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Isolatie van Mitochondriën uit Minimale hoeveelheden muis skeletspieren voor Metingen High Throughput Microplate Respiratory
Journal JoVE
Biologie
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Journal JoVE Biologie
Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements
DOI:

10:12 min

November 13, 2015

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Chapitres

  • 00:05Titre
  • 00:45Isolation of Skeletal Muscle
  • 03:47Isolation of Mitochondria from Skeletal Muscle
  • 07:50Mitochondria Membrane Integrity Assay
  • 08:42Results: Membrane Integrity Measurements of the Isolated Preparation
  • 09:31Conclusion

Summary

Traduction automatique

Here, we present a modification of a previously reported method that allows for the isolation of high quality and purified mitochondria from smaller quantities of mouse skeletal muscle. This procedure results in highly coupled mitochondria that respire with high function during microplate based respirometirc assays.

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