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Cell Sortering av neurala stamceller och progenitorceller från vuxen mus subventrikulära zonen och Live-avbildning av sina cellcykeln Dynamics
Journal JoVE
Neurosciences
This content is Free Access.
Journal JoVE Neurosciences
Cell Sorting of Neural Stem and Progenitor Cells from the Adult Mouse Subventricular Zone and Live-imaging of their Cell Cycle Dynamics
DOI:

09:27 min

September 14, 2015

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Chapitres

  • 00:05Titre
  • 01:38Basic Setup
  • 02:50SVZ Tissue Dissociation
  • 04:19Immunofluorescent Staining and Cell Sorting
  • 06:21Microscope Setup and Image Acquisition
  • 07:57Results: Live Imaging of the Cell Cycle Phases at the Level of a Single Cell
  • 08:53Conclusion

Summary

Traduction automatique

We report a Fluorescent Activated Cell Sorting (FACS)-based method to isolate neural stem cells (NSCs) and their progeny from the subventricular zone (SVZ) of the adult mouse brain. Applied to Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) transgenic mice, it allows the study of cell cycle progression by live imaging.

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