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再加上高灵敏度指纹碳水化合物自动分析模块化高通量筛选胞外多糖平台
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
DOI:

12:02 min

April 11, 2016

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Chapitres

  • 00:05Titre
  • 01:04Automated Screening of EPS-producing Strains
  • 04:41Automated Screening: Glucose-assay and Phenol-sulfuric-acid Method
  • 07:00Carbohydrate Fingerprinting: 96-well Micro Hydrolysis
  • 08:41Carbohydrate Fingerprinting: HT-PMP-derivatization
  • 10:06Results: Identification and Characterization of Microbial Exopolysaccharides in High-throughput
  • 11:02Conclusion

Summary

Traduction automatique

We present an automated modular high-throughput-method for the identification and characterization of microbial exopolysaccharides in small scale. This method combines a fast preselection to analyze the total amount of secreted polysaccharides with a detailed carbohydrate fingerprint to enable the fast screening of newly isolated bacterial strains or entire strain collections.

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