Journal
/
/
자동화 모​​듈 높은 처리량 엑소 폴리 사카 라이드 심사 플랫폼은 매우 민감한 탄수화물 지문 분석과 결합
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
DOI:

12:02 min

April 11, 2016

, ,

Chapitres

  • 00:05Titre
  • 01:04Automated Screening of EPS-producing Strains
  • 04:41Automated Screening: Glucose-assay and Phenol-sulfuric-acid Method
  • 07:00Carbohydrate Fingerprinting: 96-well Micro Hydrolysis
  • 08:41Carbohydrate Fingerprinting: HT-PMP-derivatization
  • 10:06Results: Identification and Characterization of Microbial Exopolysaccharides in High-throughput
  • 11:02Conclusion

Summary

Traduction automatique

We present an automated modular high-throughput-method for the identification and characterization of microbial exopolysaccharides in small scale. This method combines a fast preselection to analyze the total amount of secreted polysaccharides with a detailed carbohydrate fingerprint to enable the fast screening of newly isolated bacterial strains or entire strain collections.

Vidéos Connexes

Read Article