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Geomagnetico Field (Gmf) e Stabilimento Evolution: studio degli effetti di Gmf Storno su<em> Arabidopsis thaliana</em> Sviluppo e Gene Expression
Journal JoVE
Biologie du développement
This content is Free Access.
Journal JoVE Biologie du développement
Geomagnetic Field (Gmf) and Plant Evolution: Investigating the Effects of Gmf Reversal on Arabidopsis thaliana Development and Gene Expression
DOI:

11:04 min

November 30, 2015

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Chapitres

  • 00:05Titre
  • 01:14Setting of the Triaxial Coils
  • 02:01Preparation of Plant Materials and Conditions for Plant Growth
  • 05:10Quantitative Real Time-PCR (qPCR) Analysis of Arabidopsis Gene Expression
  • 08:05Results: Effects of Geomagnetic Field Reversal on Arabidopsis Morphology, Gene Expression, and Antioxidant-related Gene Expression
  • 09:23Conclusion

Summary

Traduction automatique

This protocol describes a method to test the hypothesis that the reversal of the effective geomagnetic field (GMF) induces differential gene expression and alters the morphology of Arabidopsis thaliana. A triaxial octagonal system of Helmholtz coil-pairs was used to artificially generate reversed GMF conditions in the plant growth chamber.

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