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इमेजिंग रोग प्रतिरक्षण Synapse गतिशीलता के लिए एक endothelial Planar सेल मॉडल
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
An Endothelial Planar Cell Model for Imaging Immunological Synapse Dynamics
DOI:

09:25 min

December 24, 2015

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Chapitres

  • 00:05Titre
  • 01:06Endothelia and Lymphocyte Preparation
  • 02:55Live-cell Imaging Set Up
  • 05:37Th1 Effector/Memory-endothelial Cell Immunological Synapse (IS) Initiation
  • 06:56Results: Representative Th1:Endothelial APC ISs
  • 08:51Conclusion

Summary

Traduction automatique

Adaptive immunity is controlled by dynamic 'immunological synapses' formed between T cells and antigen presenting cells. This protocol describes methods for investigating endothelial cells both as understudied physiologic APCs and as a novel type of 'planar cellular APC model'.

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