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高通量<em>斑马鱼</em>能量消耗分析
Journal JoVE
Biologie
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Journal JoVE Biologie
High Throughput Danio Rerio Energy Expenditure Assay
DOI:

08:35 min

January 27, 2016

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Chapitres

  • 00:05Titre
  • 01:01Zebrafish Breeding and Maintenance
  • 02:33Energy Expenditure Assay
  • 06:10Results: Energy Expenditure Assay Results
  • 07:28Conclusion

Summary

Traduction automatique

Zebrafish are an important model organism for the study of energy homeostasis. By utilizing a NADH2 sensitive redox indicator, alamar Blue, we have developed an assay that measures the metabolic rate of zebrafish larvae in a 96 well plate format and can be applied to drug or gene discovery.

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