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A Simple Alternative to Stereotactic Injection for Brain Specific Knockdown of miRNA
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Chapitres
- 00:00Titre
- 01:52Antagomir Design Strategy
- 02:10Antagomir-peptide Complex Preparation
- 03:08Tail Vein Injection of Antagomir-peptide Complex
- 03:29Behavioural Assays: Hind-limb Clasping
- 03:48Behavioural Assays: Ledge Test
- 04:03Behavioural Assays: Footprint Assay
- 04:32Representative Results
- 06:21Conclusion
MicroRNAs play crucial roles in the brain and are potential targets for modeling neuro-degeneration. However, perturbing miRNA levels is challenging due to the short length of miRNA and inaccessibility of the brain tissue. This video presents a method for antagomir design and brain specific delivery using a neuropeptide in mice.
