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Воображение нейтрофилов и моноцитов в брыжеечных вен Прижизненное микроскопии на наркотизированных мышей в реальном времени
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Imaging Neutrophils and Monocytes in Mesenteric Veins by Intravital Microscopy on Anaesthetized Mice in Real Time
DOI:

09:28 min

November 16, 2015

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Chapitres

  • 00:05Titre
  • 01:00Bone Marrow Single Cell Suspension Preparation
  • 02:38Neutrophil Enrichment
  • 04:18Neutrophil Labeling
  • 05:29In vivo Measurement of Neutrophil and Monocyte Behavior
  • 07:44Results: Representative Intravital Neutrophil and Monocyte Imaging
  • 08:46Conclusion

Summary

Traduction automatique

We detail a protocol to monitor the behavior of neutrophils and monocytes in mesenteric veins under steady state and inflammatory conditions using intravital confocal microscopy on anaesthetized mice.

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