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एक माउस मॉडल के मान्यकरण एक सेल विशेष तरीके में LINC परिसर बाधित करने के लिए
Journal JoVE
Biologie
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Journal JoVE Biologie
Validation of a Mouse Model to Disrupt LINC Complexes in a Cell-specific Manner
DOI:

09:02 min

December 10, 2015

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Chapitres

  • 00:05Titre
  • 01:24Preparation of Mice and Materials for Dissection and Tissue Collection
  • 02:06Dissection and Tissue Collection
  • 02:38Tissue Processing and Sectioning
  • 04:06Staining and Imaging of Cerebellum
  • 06:41Results: Disruption of Endogenous LINC Complexes in Cerebellar Purkinje Cells
  • 08:07Conclusion

Summary

Traduction automatique

Nuclear envelope proteins play a central role in many basic biological processes and have been implicated in a variety of human diseases. This protocol describes a new Cre/Lox-based mouse model that allows for the spatiotemporal control of LINC complexes disruption.

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