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Organoids som model for infektionssygdomme: Kultur i humane og murine mave Organoids og Mikroinjektion af Helicobacter pylori
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Organoids as Model for Infectious Diseases: Culture of Human and Murine Stomach Organoids and Microinjection of Helicobacter Pylori
DOI:

10:30 min

November 12, 2015

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Chapitres

  • 00:05Titre
  • 01:01Isolation and Seeding of Gastric Glands
  • 04:58Passage of Gastric Organoid Cultures
  • 06:55Microinjection of Organoid Cultures with Helicobacter pylori
  • 09:02Results: Organoid Formation and Infection with H. pylori
  • 09:48Conclusion

Summary

Traduction automatique

Stem cell derived cultures harbor tremendous potential to model infectious diseases. Here, the culture of mouse and human gastric organoids derived from adult stem cells is described. The organoids are microinjected with the gastric pathogen Helicobacter pylori.

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