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膜运输过程由单一的蛋白质解析一个高度并行的纳米孔芯片分析系统
Journal JoVE
Biologie
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Journal JoVE Biologie
Membrane Transport Processes Analyzed by a Highly Parallel Nanopore Chip System at Single Protein Resolution
DOI:

11:55 min

August 16, 2016

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Chapitres

  • 00:05Titre
  • 01:16Bio-chip Overview
  • 02:53Preparation of Large Unilamellar Vesicles (LUVs)
  • 04:45Protein Reconstitution
  • 06:14Bio-chip Holder Preparation for the Translocation Assay
  • 08:29The Translocation Assay
  • 09:47Results: Quality Control Tests and Experimental Manipulations
  • 11:28Conclusion

Summary

Traduction automatique

The presented protocol describes the analysis of membrane protein mediated transport on the single transporter level using pore-spanning solvent-free lipid bilayers. This is achieved by the creation of bulk produced nanopore array chips, combined with highly parallel data acquisition and analysis, enabling the future establishment of membrane protein effector screenings.

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