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Fluorescens Återhämtning efter Sammanfoga en droppe att mäta de tvådimensionella Spridning av en Phospholipid Mono
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Fluorescence Recovery after Merging a Droplet to Measure the Two-dimensional Diffusion of a Phospholipid Monolayer
DOI:

07:54 min

October 15, 2015

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Chapitres

  • 00:05Titre
  • 00:45Preparation of Phospholipid Monolayer at a Flat Air-water Interface
  • 02:32Preparation of Phospholipid Monolayer at the Curved Surface of a Droplet
  • 04:00Imaging Fluorescence Recovery after Droplet Merging
  • 05:17Results: Diffusion Coefficient of DOPC and DPPC Monolayer as a Function of Surface Pressure
  • 06:39Conclusion

Summary

Traduction automatique

We present a new technique to measure the lateral diffusion of a surface active species at the fluid-fluid interface by merging a droplet monolayer onto a flat monolayer.

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